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dnase treatment  (Qiagen)


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    Structured Review

    Qiagen dnase treatment
    Dnase Treatment, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 26841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnase+set/RNase-Free+DNase+Set/pmc13051718-357-1-3
    Average 99 stars, based on 26841 article reviews
    dnase treatment - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Octopamine receptors at a glance: from expression and anatomical maps to their role in development and behavior in the Drosophila melanogaster larva
    Article Snippet: Sequenced PCR products were compared to the core nucleotide database and nucleotide collection by National Cen-ter for Biotechnology Information ( ; https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=Blast-Search&LINK_LOC=blasthome ). .. Total RNA from L3 larvae was isolated using QIAzol (Qiagen, 79306) with following DNA digestion using RNase-Free DNase Set (Qiagen, 79256) and purification using RNeasy Mini Kit (Qiagen, 74104), all steps were performed according to the manufac-turers protocol. .. 250 μg RNA was reverse transcribed using SuperScriptTM III First-Strand Synthesis SuperMix for qRT-PCR (Invitrogen, 11752050).

    Article Title: IFI207 promotes antiviral responses by modulating STING ubiquitination and degradation.
    Article Snippet: HRP-conjugated antibodies (CST anti-rabbit or anti-mouse HRP IgG, 7074 and 7076, respectively) were used for detection with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific, 322209), ECL Prime Western Blotting Reagent (GE Healthcare Amersham, RPN2232), or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34,095). .. RNA was isolated by RNeasy Mini Kit (QIAGEN 74106) with on-column DNase digestion using RNase-Free DNase Set (QIAGEN 79254). cDNA was synthesized with random hexamers from the Superscript III First-Strand Synthesis System (Invitrogen 18080051). .. RT-qPCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems 4367659) with the indicated primer sets on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) (Table 1).

    Purification:

    Article Title: Octopamine receptors at a glance: from expression and anatomical maps to their role in development and behavior in the Drosophila melanogaster larva
    Article Snippet: Sequenced PCR products were compared to the core nucleotide database and nucleotide collection by National Cen-ter for Biotechnology Information ( ; https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastn&PAGE_TYPE=Blast-Search&LINK_LOC=blasthome ). .. Total RNA from L3 larvae was isolated using QIAzol (Qiagen, 79306) with following DNA digestion using RNase-Free DNase Set (Qiagen, 79256) and purification using RNeasy Mini Kit (Qiagen, 74104), all steps were performed according to the manufac-turers protocol. .. 250 μg RNA was reverse transcribed using SuperScriptTM III First-Strand Synthesis SuperMix for qRT-PCR (Invitrogen, 11752050).

    Article Title: uPAR exhibits age- and region-dependent expression in the brains of mice with Alzheimer's disease-like pathology.
    Article Snippet: .. Snap-frozen Rag-5xFAD and Rag single-hemisphere brain tissue (Supp Table 1B) was homogenized on dry ice, and total RNA was extracted using an RNAeasy Mini Kit (Qiagen, Cat. No. 74104) with a purification of DNase with RNase-Free DNase Set (Qiagen, Cat. No. 79254). .. Quality and quantity of RNA was assessed using a N60 NanoPhotometer (IMPLEN).

    other:

    Article Title: Chlamydia trachomatis deploys sphingolipids for genome organisation
    Article Snippet: Cells were transferred on ice, washed thrice with DPBS and 100 μL/well SMase lysis buffer [250 mM sucrose, 1 mM EDTA, 0.2% TX-100, 1xRoche protein inhibitor cocktail (Sigma, Cat. No. 11873580001)] were added.

    Article Title: Clinical-grade cryopreservation unlocks transplant-ready human pancreatic and stem cell–derived islets for diabetes therapy
    Article Snippet: Immunofluorescent images were captured using a Zeiss Axio Observer Microscope with ZEN v2.6 (blue edition) software.

    Synthesized:

    Article Title: IFI207 promotes antiviral responses by modulating STING ubiquitination and degradation.
    Article Snippet: HRP-conjugated antibodies (CST anti-rabbit or anti-mouse HRP IgG, 7074 and 7076, respectively) were used for detection with Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific, 322209), ECL Prime Western Blotting Reagent (GE Healthcare Amersham, RPN2232), or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, 34,095). .. RNA was isolated by RNeasy Mini Kit (QIAGEN 74106) with on-column DNase digestion using RNase-Free DNase Set (QIAGEN 79254). cDNA was synthesized with random hexamers from the Superscript III First-Strand Synthesis System (Invitrogen 18080051). .. RT-qPCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems 4367659) with the indicated primer sets on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) (Table 1).



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